Journal: Pharmaceutics

Article Title: Building of CuO 2 @Cu-TA@DSF/DHA Nanoparticle Targets MAPK Pathway to Achieve Synergetic Chemotherapy and Chemodynamic for Pancreatic Cancer Cells

doi: 10.3390/pharmaceutics16121614

Figure Lengend Snippet: Inhibitory effect of drug combination on ( A ) PANC-1 cells and ( B ) BxPC-3 cells. Cell viability of ( C ) PANC-1 cells and ( D ) BxPC-3 cells treated with different concentrations of DHA. Evaluation of single and dual drugs by double staining of live/dead cells (Calcein-AM/PI) of ( E ) PANC-1 cells and ( F ) BxPC-3 cells (×10). ( G ) ROS generation levels after treatment with PANC-1 cells and BxPC-3 cells in different dosing groups (×10). ( H ) Apoptosis of PANC-1 cells and BxPC-3 cells treated with different administration groups. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Dihydroartemisinin (DHA) and Disulfiram (DSF) were purchased from MCE (MedChemExpress, Shanghai, China); TA was purchased from Macklin; phosphate buffer solution (PBS) was purchased from Shenggong Co. Ltd. (Shanghai, China); fetal bovine serum (FBS) was bought from Biological Industries Co. (shanghai, China); total GSH, Calcein-AM/PI, apoptosis and the reactive oxygen species assay kit were purchased from Beyotime (Shanghai, China); deionized water was supplied by a Milli-Q water system.

Techniques: Double Staining

Journal: Pharmaceutics

Article Title: Building of CuO 2 @Cu-TA@DSF/DHA Nanoparticle Targets MAPK Pathway to Achieve Synergetic Chemotherapy and Chemodynamic for Pancreatic Cancer Cells

doi: 10.3390/pharmaceutics16121614

Figure Lengend Snippet: Synergistic anticancer effect of CuO 2 @Cu-TA@DSF/DHA in vitro. Fluorescence image of ( A ) PANC-1 cells and ( B ) BxPC-3 cells incubated with Rhodamine B-CCTDD NPs for 2 h and co-located with nuclear dye Hoechst33342 (×10). Different concentration treatment of CuO 2 @Cu-TA on cell viability of ( C ) PANC-1 cells and ( D ) BxPC-3 cells. The effect of different administration groups on the viability of ( E ) PANC-1 cells and ( F ) BxPC-3 cells. ( G ) Measurement of ROS fluorescence of PANC-1 cells and BxPC-3 cells after treatment with different administration groups (×10). GSH levels in ( H ) PANC-1 cells and ( I ) BxPC-3 cells after treatment in different treatment groups. ( J ) The effect of different treatment groups on the apoptosis rate of PANC-1 cells and BxPC-3 cells. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Dihydroartemisinin (DHA) and Disulfiram (DSF) were purchased from MCE (MedChemExpress, Shanghai, China); TA was purchased from Macklin; phosphate buffer solution (PBS) was purchased from Shenggong Co. Ltd. (Shanghai, China); fetal bovine serum (FBS) was bought from Biological Industries Co. (shanghai, China); total GSH, Calcein-AM/PI, apoptosis and the reactive oxygen species assay kit were purchased from Beyotime (Shanghai, China); deionized water was supplied by a Milli-Q water system.

Techniques: In Vitro, Fluorescence, Incubation, Concentration Assay